SLC29A3 Antibody

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ProSci
Product Code: PSI-8129
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8129-0.02mg0.02mg£150.00
Quantity:
PSI-8129-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
SLC29A3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

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Western blot analysis of SLC29A3 in mouse bladder tissue lysate with SLC29A3 antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of SLC29A3 in rat colon tissue with SLC29A3 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of SLC29A3 in rat colon muscle tissue with SLC29A3 antibody at 20 μg/mL.
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Western blot analysis of SLC29A3 in mouse bladder tissue lysate with SLC29A3 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of SLC29A3 in rat colon tissue with SLC29A3 antibody at 5 μg/mL.
Immunofluorescence of SLC29A3 in rat colon muscle tissue with SLC29A3 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
Solute carrier family 29 member 3, Equilibrative nucleoside transporter 3, ENT3, HCLAP, HJCD, PHID
Application Note:
SLC29A3 antibody can be used for detection of SLC29A3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
SLC29A3 is a member of the equilibrative nucleoside transporter family which plays a key role in nucleoside and nucleobase uptake for salvage pathways of nucleotide synthesis (1,2). SLC29A3 is a transmembrane glycoprotein that localizes to the lysosomal membrane and is a broad selectivity, low affinity nucleoside transporter (3). Mutations in the SLC29A3 gene have been associated with H syndrome, which is characterized by cutaneous hyperpigmentation and hypertrichosis, hepatosplenomegaly, heart anomalies, and hypogonadism (4). A related disorder, PHID (pigmented hypertrichosis with insulin-dependent diabetes mellitus), has also been associated with mutations at this locus (5).
Background References:
  • Hyde RJ, Cass CE, Young JD, et al. The ENT family of eukaryotic nucleoside and nucleobase transporters: recent advances in the investigation of structure/function relationships and the identification of novel isoforms. Mol. Membr. Biol. 2001; 18:53-63.
  • Young JD, Yao SY, Baldwin JM, et al. The human concentrative and equilibrative nucleoside transporter families, SLC28 and SLC29. Mol. Aspects. Med. 34:529-47.
  • Baldwin SA, Yao SY, Hyde RJ, et al. Functional characterization of novel human and mouse equilibrative nucleoside transporters (hENT3 and mENT3) located in intracellular membranes. J. Biol. Chem. 2005; 280:15880-7.
  • Priya TP, Philip N, Molho-Pessach V, et al. H syndrome: novel and recurrent mutations in SLC29A3. Br. J. Dermatol. 2010; 162:1132-4.
Buffer:
SLC29A3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (95%)
Immunogen:
SLC29A3 antibody was raised against a 19 amino acid peptide near the amino terminus of human SLC29A3.

The immunogen is located within the first 50 amino acids of SLC29A3.
NCBI Gene ID #:
55315
NCBI Official Name:
solute carrier family 29 (equilibrative nucleoside transporter), member 3
NCBI Official Symbol:
SLC29A3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 52 kDa

Observed: 51 kDa
Protein Accession #:
NP_060814
Protein GI Number:
148596922
Purification:
SLC29A3 antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Homeostasis
SPECIFICITY:
SLC29A3 antibody is human, mouse and rat reactive. At least two isoforms of SLC29A3 are known to exist; this antibody will detect both isoforms. SLC29A3 antibody is predicted to not cross-react with other SLC29 proteins.
Swissprot #:
Q9BZD2
User NOte:
Optimal dilutions for each application to be determined by the researcher.