SNRPN Antibody

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ProSci
Product Code: PSI-8155
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8155-0.02mg0.02mg£150.00
Quantity:
PSI-8155-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
SNRPN antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

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Western blot analysis of SNRPN in THP-1 cell lysate with SNRPN antibody at (A) 0.5 and (B) 1 μg/ml.
2 / 3
Immunohistochemistry of SNRPN in human spleen tissue with SNRPN antibody at 5 μg/mL.
3 / 3
Immunofluorescence of SNRPN in human spleen tissue with SNRPN antibody at 20 μg/mL.
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Western blot analysis of SNRPN in THP-1 cell lysate with SNRPN antibody at (A) 0.5 and (B) 1 μg/ml.
Immunohistochemistry of SNRPN in human spleen tissue with SNRPN antibody at 5 μg/mL.
Immunofluorescence of SNRPN in human spleen tissue with SNRPN antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
Small nuclear ribonucleoprotein-associated protein N, HCERN3, PWCR, RT-LI, SM-D, SMN, SNRNP-N, SNURF-SNRPN, sm-N
Application Note:
SNRPN antibody can be used for detection of SNRPN by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The small nuclear riboprotein-associated protein N (SNRPN) is one polypeptide of a small nuclear ribonucleoprotein complex and belongs to the snRNP SMB/SMN family. SNRPN plays a role in pre-mRNA processing and alternative splicing (1). SNRPN arises from a bicistronic transcript that also encodes a protein identified as the SNRPN upstream reading frame (SNURF) (2). Mutations or deletions in the SNRPN gene may be linked to responsible for Angelman syndrome or Prader-Willi syndrome (3).
Background References:
  • Ozcelik T, Leff S, Robinson W, et al. Small nuclear ribonucleoprotein polypeptide N (SNRPN), an expressed gene in the Prader-Willi syndrome critical region. Nat. Genet. 1992; 2:265-9.
  • Gray TA, Saitoh S, and Nicholls RD. An imprinted, mammalian bicistronic transcript encodes two independent proteins. Proc. Natl. Acad. Sci. USA 1999; 96:5616-21.
  • Chen Y, Liu YJ, Pei YF, et al. Copy number variations at the Prader-Willi syndrome region on chromosome 15 and associations with obesity in whites. Obesity 2011; 19:1229-34.
Buffer:
SNRPN antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (95%)
Immunogen:
SNRPN antibody was raised against a 20 amino acid peptide near the amino terminus of human SNRPN.

The immunogen is located within amino acids 30 - 80 of SNRPN.
NCBI Gene ID #:
6638
NCBI Official Name:
small nuclear ribonucleoprotein polypeptide N
NCBI Official Symbol:
SNRPN
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 26 kDa

Observed: 25 kDa
Protein Accession #:
NP_003088
Protein GI Number:
4507135
Purification:
SNRPN antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
SNRPN antibody is human, mouse and rat reactive.
Swissprot #:
P63164
User NOte:
Optimal dilutions for each application to be determined by the researcher.