TRB3 Antibody
| Code | Size | Price |
|---|
| PSI-8099-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8099-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
TRB3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Tribbles homolog 3, tribbles pseudokinase 3, TRIB3, NIPK, SINK, SKIP3, C20orf97
Application Note:
TRB3 antibody can be used for detection of TRB3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
TRB3 was initially identified as a novel kinase-like gene that was overexpressed in multiple human tumors and regulated by hypoxia (1). In the liver, TRB3 binds and inhibits AKT activity, leading to impaired insulin signaling (2), whereas in adipose tissue TRB3 is involved in the regulation of fatty acid oxidation through ubiquitination of Acetyl-CoA carboxylase (3). Endoplasmic reticulum (ER) stress induces TRB3 expression in skeletal muscle, leading to a decrease in insulin signaling and glucose uptake, suggesting that inhibition of TRB3 expression may be a potential therapeutic treatment for managing insulin resistance (4).
Background References:
- Bowers AJ, Scully S, and Boylan JF. SKIP3, a novel drosophila tribbles ortholog, is overexpressed in human tumors and is regulated by hypoxia. Oncogene 2003; 22:2823-35.
- Du K, Herzig S, Kulkarni RN, et al. TRB3: a tribbles homolog that inhibits Akt/PKB activation by insulin in liver. Science 2003; 300:1574-7.
- Qi L, Heredia JE, Altarejos JY, et al. TRB3 inks the E3 ubiquitin ligase COP1 to lipid metabolism. Science 2006; 312:1763-6.
- Koh HJ, Toyoda T, Didesch MM, et al. Tribbles 3 mediates endoplasmic reticulum stress-induced insulin resistance in skeletal muscle. Nat. Commun. 2013; 4:1871.
Buffer:
TRB3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (76%)
Immunogen:
TRB3 antibody was raised against a 17 amino acid peptide near the center of human TRB3.
The immunogen is located within amino acids 100 - 150 of TRB3.
The immunogen is located within amino acids 100 - 150 of TRB3.
NCBI Gene ID #:
57761
NCBI Official Name:
tribbles pseudokinase 3
NCBI Official Symbol:
TRB3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 39 kDa
Observed: 39 kDa
Observed: 39 kDa
Protein Accession #:
NP_066981
Protein GI Number:
31542265
Purification:
TRB3 antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Signal Transduction
SPECIFICITY:
TRB3 antibody is human, mouse and rat reactive. At least two isoforms of TRB3 are known to exist; this antibody will detect both isoforms.
Swissprot #:
Q96RU7
User NOte:
Optimal dilutions for each application to be determined by the researcher.