Vinculin Antibody [8B5]

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ProSci
Product Code: PSI-PM-7813
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-PM-7813-0.02mg0.02mg£150.00
Quantity:
PSI-PM-7813-0.1mg0.1mg£515.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species:
  • Chicken (Gallus)
  • Human
  • Mouse
  • Puumala Virus
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Vinculin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

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Western blot analysis of Vinculin in 293, A431, A549, HeLa, HepG2, K562, 3T3, Raji, U937 cell lysate and human kidney, human breast, mouse brain, mouse lung, mouse spleen, rat heart, rat lung, rat spleen, rat liver, rabbit spleen, rabbit brain and chicken spleen tissue lysate with Vinculin antibody at 1 μg/ml.
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Western blot analysis of Vinculin in 293, A431, A549, HeLa, HepG2, K562, 3T3, Raji, U937 cell lysate and human kidney, human breast, mouse brain, mouse lung, mouse spleen, rat heart, rat lung, rat spleen, rat liver, rabbit spleen, rabbit brain and chicken spleen tissue lysate with Vinculin antibody at 1 μg/ml.

Documents

Further Information

Additional Names:
CMD1W, CMH15, Metavinculin, MVCL
Application Note:
Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 μg/ml.

Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Background:
Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).
Background References:
  • Burridge et al. Annu. Rev. Cell Biol.1988; 4:487-525.
  • Gilmore et al. J. Cell Sci. 1992; 103:719-31.
  • Deakin et al. PLoS One 2012; 7:e37990.
  • Goldmann et al. Cell Biol. Int. 2013; Feb 1.
Buffer:
Vinculin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Vinculin antibody was raised against a 23 amino acid peptide near the amino terminus of human Vinculin.
NCBI Gene ID #:
7414
NCBI Official Name:
vinculin
NCBI Official Symbol:
VCL
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 117 kDa

Observed: 120 kDa
Protein Accession #:
NP_003364
Protein GI Number:
4507877
Purification:
Vinculin antibody is purified via Protein A prification.
Research Area:
Homeostasis
SPECIFICITY:
Vinculin antibody is human, mouse, rat, rabbit and chicken reactive. At least three isoforms of Vinculin are known to exist.
User NOte:
Optimal dilutions for each application to be determined by the researcher.