beta-Actin Antibody [10B7]

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ProSci
Product Code: PSI-PM-7547
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-PM-7547-0.02mg0.02mg£150.00
Quantity:
PSI-PM-7547-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species:
  • Chicken (Gallus)
  • Drosophila (fruit fly)
  • Human
  • Mouse
  • Puumala Virus
  • Rat
  • Zebrafish
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
beta-Actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot of beta-Actin in 293, A431, HepG2, Jurkat, K562, MOLT, 3T3, Raji, Ramos, THP-1, U937, human brain, mouse brain, rat brain, rabbit brain, rat liver, rabbit spleen, chicken liver, chicken small intestine, zebrafish, and drosophila lysate at 1 μg/mL.
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Western blot of beta-Actin in 293, A431, HepG2, Jurkat, K562, MOLT, 3T3, Raji, Ramos, THP-1, U937, human brain, mouse brain, rat brain, rabbit brain, rat liver, rabbit spleen, chicken liver, chicken small intestine, zebrafish, and drosophila lysate at 1 μg/mL.

Documents

Further Information

Additional Names:
beta-Actin Antibody: Beta actin, b actin
Application Note:
b-actin antibody can be used for the detection of b-actin by Western blot at 0.5 - 2 μg/mL.

Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Background:
Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Background References:
  • Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol. 2004; 36:1890-909.
  • Gourlay CW and Ayscough KR. The actin cytoskeleton: a key regulator of apoptosis and ageing. Nat. Rev. 2005; 6:583-9.
Buffer:
beta-Actin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
b-actin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human b-actin.
NCBI Gene ID #:
60
NCBI Official Name:
actin, beta
NCBI Official Symbol:
ACTB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 42 kDa

Observed: 44 kDa
Protein Accession #:
AAH02409
Protein GI Number:
12803203
Purification:
beta-Actin Antibody is Protein G purified.
Research Area:
Homeostasis
SPECIFICITY:
beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.
User NOte:
Optimal dilutions for each application to be determined by the researcher.