CDK7 Antibody
| Code | Size | Price |
|---|
| PSI-31-031-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4°C. For longer periods of storage, store CDK7 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
CDK7, CAK1, HCAK, MO15, STK1, CDKN7, p39MO15
Application Note:
CDK7 antibody can be used for detection of CDK7 by ELISA at 1:75000. CDK7 antibody can be used for detection of CDK7 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
Cdk7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive carboxyl-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Cdk7 is involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Background References:
- Tassan, J.P., et al., (1994) J. Cell Biol. 127:467-478..
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CDK7.
NCBI Gene ID #:
1022
NCBI Official Name:
cyclin-dependent kinase 7
NCBI Official Symbol:
CDK7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
NP_001790
Protein GI Number:
4502743
Purification:
Antibody is purified by Protein A/G affinity chromatography method.
Research Area:
Transcription ,Cell Cycle ,Cancer ,Signal Transduction
Swissprot #:
P50613
User NOte:
Optimal dilutions for each application to be determined by the researcher.