GFP Antibody
| Code | Size | Price |
|---|
| PSI-XR-9001-0.1mg | 0.1mg | £577.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store vial at -20? C or below prior to opening. Dilute only prior to immediate use. Forextended storage, aliquot contents and freeze at -20? C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.
Documents
Further Information
Additional Names:
Green fluorescent protein
Application Note:
Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Recommended dilutions are as follows, ELISA 1:15,000 - 1:60,000, Western Blot 1:500 - 1:5,000, Immunohistochemistry 1:200 - 1:3,000. (Optimal dilutions should be determined by user).
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% (w/v) Sodium Azide; pH 7.2
Concentration:
1.0 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
NCBI Gene ID #:
7011691
NCBI Official Name:
green fluorescent protein
NCBI Official Symbol:
gfp
NCBI Organism:
Neisseria gonorrhoeae
Physical State:
Liquid
Protein Accession #:
YP_002302326.1
Protein GI Number:
211909965
Purification:
Anti-GFP Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.
Research Area:
Other
Swissprot #:
P42212
User NOte:
Optimal dilutions for each application to be determined by the researcher.