ChAT Peptide
| Code | Size | Price |
|---|
| PSI-45-038P-0.1mg | 0.1mg | £404.00 |
Quantity:
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Overview
Regulatory Status: RUO
Target Species: Human
Application: Blocking
Storage:
-20°C
Documents
Further Information
Additional Names:
CMS1A, CMS1A2, CHOACTASE, Choline O-acetyltransferase, CHOACTase
Application Note:
ChAT Peptide is used for blocking the activity of ChAT antibody (catalog number 45-038). The ChAT antibody ships at 0.5 mg/mL and the ChAT Peptide ships as a 100 ug pellet. When peptide is reconstituted in 200 uL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
Buffer:
ChAT peptide is supplied as 100 μg of dried peptide. When the peptide is reconstituted in 200 μL water, the concentration will be 0.5 mg/mL.
NCBI Gene ID #:
1103
NCBI Official Name:
choline O-acetyltransferase
NCBI Official Symbol:
CHAT
NCBI Organism:
Homo sapiens
Peptide aa Location:
ChAT peptide corresponds to 14 amino acids near the C-terminus region of ChAT.
Physical State:
Lyophilized
Protein Accession #:
NP_065574, NP_066264, NP_066265, NP_066266
Protein GI Number:
188595670
Swissprot #:
P28329