AIF4 Peptide
| Code | Size | Price |
|---|
| PSI-45-222P-0.1mg | 0.1mg | £404.00 |
Quantity:
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Overview
Regulatory Status: RUO
Target Species: Human
Application: Blocking
Storage:
-20°C
Documents
Further Information
Additional Names:
AIF4, AIP4, NAPP1, dJ468O1.1, E3 ubiquitin-protein ligase Itchy homolog, Atrophin-1-interacting protein 4, Itch
Application Note:
AIF4 Peptide is used for blocking the activity of AIF4 antibody (catalog number 45-222). The AIF4 antibody ships at 0.5 mg/mL and the AIF4 Peptide ships as a 100 ug pellet. When peptide is reconstituted in 200 uL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
Buffer:
AIF4 peptide is supplied as 100 μg of dried peptide. When the peptide is reconstituted in 200 μL water, the concentration will be 0.5 mg/mL.
NCBI Gene ID #:
83737
NCBI Official Name:
itchy E3 ubiquitin protein ligase
NCBI Official Symbol:
ITCH
NCBI Organism:
Homo sapiens
Peptide aa Location:
AIF4 peptide corresponds to 13 amino acids of AIF4.
Physical State:
Lyophilized
Protein Accession #:
NP_113671.3
Protein GI Number:
27477109
Swissprot #:
Q96J02