SIM2 Peptide
| Code | Size | Price |
|---|
| PSI-42-710P-0.1mg | 0.1mg | £404.00 |
Quantity:
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Overview
Regulatory Status: RUO
Target Species: Mouse
Application: Blocking
Storage:
-20°C
Documents
Further Information
Additional Names:
SIM, bHLHe15, HMC13F06, HMC29C01, BHLHE15, Single-minded homolog 2, Class E basic helix-loop-helix protein 15
Application Note:
SIM2 Peptide is used for blocking the activity of SIM2 antibody (catalog number 42-710). The SIM2 antibody ships at 0.5 mg/mL and the SIM2 Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
Buffer:
SIM2 peptide is supplied as 100 μg of dried peptide. When the peptide is reconstituted in 200 μL water, the concentration will be 0.5 mg/mL.
NCBI Gene ID #:
6493
NCBI Official Name:
single-minded family bHLH transcription factor 2
NCBI Official Symbol:
SIM2
NCBI Organism:
Homo sapiens
Peptide aa Location:
SIM2 peptide is a synthetic peptide corresponding to the internal region of SIM2.
Physical State:
Lyophilized
Protein Accession #:
NP_005060.1; NP_033664.2
Protein GI Number:
4827004
Swissprot #:
Q14190