MIA Recombinant Protein
| Code | Size | Price |
|---|
| PSI-40-226-0.005mg | 0.005mg | £300.00 |
Quantity:
| PSI-40-226-0.02mg | 0.02mg | £428.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Storage:
The lyophilized MIA recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted MIA is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing
Documents
Further Information
Additional Names:
CD-RAP, Melanoma-derived growth regulatory protein, Melanoma inhibitory activity protein
Background:
MIA is the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family; MIA, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. MIA is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of MIA may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis. Recombinant human MIA is a 12.2 kDa globular protein containing 108 amino acid residues including two intramolecular disulfide bonds.
Biological Activity:
The ED50 was determined by the dose - dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4 - 6 μg/mL.
NCBI Gene ID #:
8190
NCBI Official Name:
melanoma inhibitory activity
NCBI Official Symbol:
MIA
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
NP_001189482.1
Protein GI Number:
321400133
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Recombinant Protein Sequence:
#N/A
Source:
E. coli
Swissprot #:
Q16674