Persephin Recombinant Protein
| Code | Size | Price |
|---|
| PSI-40-501-0.005mg | 0.005mg | £300.00 |
Quantity:
| PSI-40-501-0.02mg | 0.02mg | £428.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Storage:
The lyophilized Persephin recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted Persephin is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protei
Documents
Further Information
Additional Names:
PSP, Persephin, PSP
Background:
Persephin is a disulfide-linked homodimer neurotrophic factor structurally related to GDNF, Artemin, and Neurturin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Persephin signals through a multicomponent receptor system, composed of RET and one of four GFR alpha (alpha1-alpha4) receptors. The GFRalpha4 was first identified in chicken and was later shown to be the preferential binding subunit for Persephin. Persephin promotes the survival of ventral midbrain dompaminergic neurons and motor neurons after sciatic nerve oxotomy, and like GNDF, promotes ureteric bud branching. However, in contrast to GDNF and Neurturin, Persephin does not support survival of peripheral neurons. Recombinant human Persephin is a disulfide-linked homodimer, composed of two 10.3 kDa polypeptide chains (192 total amino acid residues). Each chain contains seven conserved cysteine residues, one of which (Cys 63) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration. Manufactured under license from the University of Washington patent# US 6,716,600; US 6,692,943; US 6,645,937; US 6,403,335; US 6,232,449; US 6,222,022.
Biological Activity:
Human Persephin induces RET phosphorylation using a concentration range of 0.1 - 1.0 ng/mL. Human Persephin binds to mammalian GFRa4 with the Kd of 100pM. Other members of the GDNF family (Artemin, GDNF and Neurturin) do not bind to mammalian GFRa4.
NCBI Gene ID #:
5623
NCBI Official Name:
persephin
NCBI Official Symbol:
PSPN
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
NP_004149.1
Protein GI Number:
4758974
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Recombinant Protein Sequence:
#N/A
Source:
E. coli
Swissprot #:
O60542