Lin28-TAT Recombinant Protein
| Code | Size | Price |
|---|
| PSI-40-562-0.005mg | 0.005mg | £300.00 |
Quantity:
| PSI-40-562-0.025mg | 0.025mg | £428.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Storage:
The lyophilized Lin28-TAT recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted Lin28-TAT stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein,
Documents
Further Information
Additional Names:
CSDD1, LIN28, LIN-28, ZCCHC1, lin-28A, CSDD1, Protein lin-28 homolog A, Zinc finger CCHC domain-containing protein 1, Lin-28A
Background:
Lin28 is a RNA-binding protein that belongs to a diverse family of structurally-related transcription factors. Lin28 is found abundantly in embryonic stem cells (ESCs), and to a lesser extent in placenta and testis. Lin28 has been shown to block let-7 microRNA processing and maturation, a necessary step in the differentiation of stem cells and certain cancer cell lines. Together with Sox2, Oct4, and Nanog, Lin28 can induce the reprogramming of primary human fibroblasts to a pluripotent state. Lin28 and other regulatory proteins can be introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing proteins into primary as well as transformed cells. Recombinant human Lin28-TAT is a 24.4 kDa protein containing 222 amino acid residues, including 13- residue C-terminal TAT peptide.
Biological Activity:
Biological activity was measured by its ability to induce fluorescence in Lin28 reporter cells (293 cells transfected with fluorescent protein genes under Lin28 control). Optimum activity was achieved at 20 μg/mL after incubation for 72 hr. Endotoxin level is less than 0.1 ng per μg (1EU/μg).
NCBI Gene ID #:
79727
NCBI Official Name:
lin-28 homolog A (C. elegans)
NCBI Official Symbol:
LIN28A
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
NP_078950.1
Protein GI Number:
13375938
Purity:
Greater than 90% by SDS-PAGE gel and HPLC analyses.
Recombinant Protein Sequence:
#N/A
Source:
E. coli
Swissprot #:
Q9H9Z2