SHH Recombinant Protein
| Code | Size | Price |
|---|
| PSI-40-188-0.005mg | 0.005mg | £300.00 |
Quantity:
| PSI-40-188-0.025mg | 0.025mg | £428.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Storage:
The lyophilized SHH recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted SHH is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing
Documents
Further Information
Additional Names:
TPT, HHG1, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5, Sonic hedgehog protein, HHG-1, SHH
Background:
Members of the Hedgehog (Hh) family are highly conserved proteins which are widely represented throughout the animal kingdom. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) are structurally related and share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical). The biologically active form of Hh molecules is obtained by autocatalytic cleavage of their precursor proteins and corresponds to approximately the N-terminal one half of the precursor molecule. Although Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they use the same signaling pathway and can substitute for each other in experimental systems. Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.
Biological Activity:
Biological activity was determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL - 226) cells. The expected ED50 for this effect is 0.8 - 1.0 μg/mL.
NCBI Gene ID #:
6469
NCBI Official Name:
sonic hedgehog
NCBI Official Symbol:
SHH
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
NP_000184.1
Protein GI Number:
4506939
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Recombinant Protein Sequence:
#N/A
Source:
E. coli
Swissprot #:
Q15465