TIGAR-TAT Recombinant Protein
| Code | Size | Price |
|---|
| PSI-40-575-0.005mg | 0.005mg | £300.00 |
Quantity:
| PSI-40-575-0.025mg | 0.025mg | £428.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Storage:
The lyophilized TIGAR-TAT recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted TIGAR-TAT stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein,
Documents
Further Information
Additional Names:
FR2BP, TIGAR, C12orf5, Fructose-2, 6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator
Background:
TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2-6 bisphosphate (F-2-6-BP) to fructose-6-phosphate and inorganic phosphate. F-2-6-BP is a powerful activator of 6-phosphofructose-1 kinase, the rate limiting enzyme of glycolysis. By lowering the intracellular level of F-2-6-BP, TIGAR expression leads to increased glucose processing via the pentose phosphate pathway, the major cellular source for NADPH. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other intracellular proteins into primary as well as transformed cells. Recombinant human TIGAR-TAT expressed in E. coli is a 36 kDa protein containing 284 amino-acid residues, including the 271 residues of full-length TIGAR fused to a 13-residue C-terminal peptide containing the TAT transduction domain (GGYGRKKRRQRRR).
Biological Activity:
Pretreatment with TIGAR-TAT for 4 hrs, using a concentration range 0.1-5.0 μg/mL, protects U2OS cells from apoptosis induced by hydrogen peroxide. Endotoxin level is less than 0.1 ng per μg (1EU/μg).
NCBI Gene ID #:
57103
NCBI Official Name:
chromosome 12 open reading frame 5
NCBI Official Symbol:
C12orf5
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
NP_065108.1
Protein GI Number:
9966849
Purity:
Greater than 95% by SDS-PAGE gel and HPLC analyses.
Recombinant Protein Sequence:
#N/A
Source:
E. coli
Swissprot #:
Q9NQ88