Visfatin Recombinant Protein
| Code | Size | Price |
|---|
| PSI-40-111-0.005mg | 0.005mg | £300.00 |
Quantity:
| PSI-40-111-0.025mg | 0.025mg | £428.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Storage:
The lyophilized Visfatin recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted Visfatin is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein,
Documents
Further Information
Additional Names:
VF, PBEF, PBEF1, VISFATIN, 1110035O14Rik, Nicotinamide phosphoribosyltransferase, Pre-B-cell colony-enhancing factor 1, NAmPRTase
Background:
Visfatin is a 55 kDa protein produced and secreted primarily by white adipose tissue. Recently, Visfatin was isolated from visceral fat deposits and shown to possess insulin-mimetic activity. Like insulin, Visfatin exerts hypoglycemic effects by interacting with the insulin receptor. The binding affinity of Visfatin for the insulin receptor is similar to that of insulin, but it does not compete with insulin, suggesting that the two proteins interact with different receptor sites. The circulating levels of Visfatin are much lower than those of insulin and are not affected by feeding, implying that the hypoglycemic effect of Visfatin may not be of physiological importance. The plasma Visfatin levels, like those of Leptin, correlate positively with the percent of body fat and increase during the development of obesity. Another similarity between Visfatin and Leptin is that their amino acid sequence is highly conserved across different mammalian species and shows no homology to any known protein. Receptors for both Leptin (Ob-R) and Visfatin (i.e. the insulin receptor) are expressed by neurons within the arcuate nucleus of the hypothalamus, a brain area that plays a pivotal role in the regulation of energy metabolism. Although the metabolic function of Visfatin is still unknown, it appears that this newly identified adipocytokine might play an important role, similar to that of Leptin, in the regulation of body weight, i.e. as an afferent signal reflecting excess body fat. Recombinant human Visfatin is a 52.5 kDa protein containing 465 amino acid residues (isoform 1).
Biological Activity:
The ED50 was determined by the dose - dependant proliferation of the RPMI 8226 cells. The expected ED50 for this effect is 15.0 - 20.0 ng/mL.
NCBI Gene ID #:
10135
NCBI Official Name:
nicotinamide phosphoribosyltransferase
NCBI Official Symbol:
NAMPT
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
NP_005737.1
Protein GI Number:
5031977
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Recombinant Protein Sequence:
#N/A
Source:
E. coli
Swissprot #:
P43490