PAF-AH Recombinant Protein
| Code | Size | Price |
|---|
| PSI-40-128-0.005mg | 0.005mg | £300.00 |
Quantity:
| PSI-40-128-0.02mg | 0.02mg | £428.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Storage:
The lyophilized PAF-AH recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted PAF-AH is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exp
Documents
Further Information
Additional Names:
PAFAD, PAFAH, LP-PLA2, LDL-PLA2, Platelet-activating factor acetylhydrolase, 1-alkyl-2-acetylglycerophosphocholine esterase, PAF acetylhydrolase
Background:
Platelet Activating Factor (PAF) is a biologically active phospholipid, which exerts primarily proinflammatory activities by specifically signaling through G-protein-coupled receptors on platelets, neutrophils, and monocytes. Platelet Activating Factor Acetylhydrolase (PAF-AH) is a secreted protein that mediates PAF activity by specifically catalyzing hydrolysis of the "sn2? ester bond, resulting in the conversion of PAF to the biologically inactive lyso-PAF. PAF-AH can also interact with LDL particles to induce the hydrolysis of LDL associated, oxidized phospholipids, generating lysophosphatidylcholine (lyso-PC) and other lysophospholipids. Recombinant PAF-AH is a 420 amino acid glycoprotein which migrates with an apparent molecular mass of 47-55 kDa by SDS-PAGE analysis.
Biological Activity:
Biological activity was measured by its ability to cleave a PAF analog in a chromogenic substrate linked assay. At a PAF - AH concentration of 10.0 μg/mL, 50% cleavage was achieved at an incubation time of approximately 2 minutes.
NCBI Gene ID #:
7941
NCBI Official Name:
phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
NCBI Official Symbol:
PLA2G7
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
NP_001161829.1
Protein GI Number:
270133071
Purity:
Greater than 95% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Recombinant Protein Sequence:
#N/A
Source:
HEK293 cells
Swissprot #:
Q13093