EndoG Fragment Recombinant Protein

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ProSci
Product Code: PSI-95-103
Product Group: Recombinant Proteins
Supplier: ProSci
CodeSizePrice
PSI-95-103-0.05mg0.05mg£347.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Mass Spectrometry
  • Western Blot (WB)
Storage:
Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Documents

Further Information

Additional Names:
EndoG Antibody : Endo G
Application Note:
This recombinant protein can be used for WB and ELISA. For research use only.
Background:
The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribo-nuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1-3). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndoG also cleaves DNA in vitro (4).
Background References:
  • Li LY, Luo X, Wang X. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature 2001; 412:95-9.
  • Parrish J, Li L, Klotz K, et al. Mitochondrial endonuclease G is important for apoptosis in C. elegans . Nature 2001; 412:90-4
  • Hengartner MO. Apoptosis. DNA destroyers. Nature 2001; 412:27, 29.
  • Widlak P, Li LY, Wang X, et al. Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I. J. Biol. Chem. 2001; 276:48404-9.
Buffer:
1X PBS containing 0.1% SDS and 0.02% NaN3
Fusion Tag:
Fusion Partner: His-tag and strepII-tag at N-terminus
NCBI Gene ID #:
2021
NCBI Official Name:
endonuclease G
NCBI Official Symbol:
ENDOG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Predicted Molecular Weight:
15 kDa (Calculated)
Protein Accession #:
Q14249
Protein GI Number:
24638471
Purity:
~95%
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
Q14249