XBP1 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-95-109-0.05mg | 0.05mg | £347.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Mass Spectrometry
- Neutralisation
- Western Blot (WB)
Storage:
Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Documents
Further Information
Additional Names:
XBP-1 Antibody : XBP2, TREB5, XBP-1
Application Note:
This recombinant protein can be used for WB and ELISA. For research use only.
Background:
X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER) (1). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94 (3,4).
Background References:
- Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91.
- Calfon M, Zeng H, Urano F, et al. IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA. Nature 2002; 415:92-6.
- Haze K, Yoshida H, Yanagi H, et al. Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic stress. Mol. Cell. Biol. 1999; 10:3787-99.
- Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.
Buffer:
1X PBS containing 0.1% SDS
Fusion Tag:
Fusion Partner: N-terminal His-tag
NCBI Gene ID #:
7494
NCBI Official Name:
X-box binding protein 1
NCBI Official Symbol:
XBP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Predicted Molecular Weight:
25 kDa (Calculated)
Protein Accession #:
BAB82982
Protein GI Number:
18148382
Purity:
~95%
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P17861