Integrin alpha 4 Recombinant Protein

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ProSci
Product Code: PSI-95-110
Product Group: Recombinant Proteins
Supplier: ProSci
CodeSizePrice
PSI-95-110-0.05mg0.05mg£347.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Mass Spectrometry
  • Neutralisation
  • Western Blot (WB)
Storage:
Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Documents

Further Information

Additional Names:
Integrin alpha 4 Antibody: IA4, CD49D, Integrin alpha-4, CD49 antigen-like family member D
Application Note:
This recombinant protein can be used for WB and ELISA. For research use only.
Background:
The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains (reviewed in 1). Alpha 4 (4) chain associates with either beta 1 (1) or beta 7 (7) chain. It has been demonstrated that the putative ligand-binding sites of both integrin 41 and 47 is located on the 4 chain. These ligands included Madcam, VCAM, and fibronectin (2-4). Madcam is known as the principal ligand for integrin a4b7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin 47, the gut mucosal homing receptor for peripheral T cells (5). This recombinant protein fragment includes the ligand and monoclonal antibody binding domains; this binding has been tested and verified in ELISA assays. This recombinant protein is recognized PSI antibodies Integrin 4 (IN1) (PSI 4827) and Integrin 4 (IN2) (PSI 4837), but not Integrin 4 (CT) (PSI 4783).
Background References:
  • Wegener KL and Campbell ID. Transmembrane and cytoplasmic domains in integrin activation and protein-protein interactions. Mol. Membr. Biol. 2008; 25:376-87.
  • Kamata T, Puzon W, and Takada Y. Identification of putative ligand-binding sites of the integrin alpha 4 beta 1 (VLA-4, CD49d/CD29). Biochem J. 1995; 305:945-51.
  • Berlin C, Berg EL, Briskin MJ, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 1993; 74:185-95.
  • Pulido R, Elices MJ, Campanero MR, et al. Functional evidence for three distinct and independently inhibitable adhesion activities mediated by the human integrin VLA-4. Correlation with distinct alpha 4 epitopes. J. Biol. Chem. 1991; 266:10241-5.
Buffer:
50mM Tris pH7.5, 150mM NaCl, 5 mM MgCl2
Fusion Tag:
Fusion Partner: N-terminal His-tag
NCBI Gene ID #:
3676
NCBI Official Name:
integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
NCBI Official Symbol:
ITGA4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Predicted Molecular Weight:
17 kDa (Calculated)
Protein Accession #:
NP_000876
Protein GI Number:
67191027
Purity:
~95%
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P13612