Alkaline Phosphatase Rabbit Anti-Chicken IgY (H&L) Antibody

ProSci
Product Code: PSI-XR-9180
Product Group: Secondary Antibody
Supplier: ProSci
CodeSizePrice
PSI-XR-9180-1mg1mg£705.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgY
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Chicken (Gallus)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY can be stored vial at 4 °C before opening. DO NOT FREEZE. This product is stable at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase c

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Further Information

Application Note:
Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 mg of Chicken IgY in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 is suggested for this product. (Optimal dilution should be determined by user).
Buffer:
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0; 0.1% (w/v) Sodium Azide; 10 mg/mL Bovine Serum Albumin (BSA)
Conjugate:
Alkaline Phosphatase
Immunogen:
Chicken IgY - whole molecule
Physical State:
Liquid
Purification:
Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgY coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgY and Chicken Serum.