Peroxidase Rabbit Anti-Goat IgG (H&L) Antibody

ProSci
Product Code: PSI-XR-9320
Product Group: Secondary Antibody
Supplier: ProSci
CodeSizePrice
PSI-XR-9320-1mg1mg£669.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Caprine (goat)
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Peroxidase Conjugated Affinity Purified anti-Goat IgG can be stored vial at 4 °C prior to restoration. Resuspend with 1.0 ml of deionized water (or equivalent. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of fr

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Further Information

Application Note:
Peroxidase Conjugated Affinity Purified anti-Goat IgG is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:14,000 - 1:60,000, Western Blot 1:1,000 - 1:5,000, Immunohistochemistry 1:500 - 1:2,500. (Optimal dilution should be determined by user).
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/mL Bovine Serum Albumin (BSA); 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Conjugate:
HRP
Immunogen:
Goat IgG - whole molecule
Physical State:
Lyophilized
Purification:
Peroxidase Conjugated Affinity Purified anti-Goat IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human Serum Proteins.
Specificity:
Minimum Cross reactivity to Human Serum Proteins