Peroxidase Goat Anti-Mouse IgG (H&L) Antibody

ProSci
Product Code: PSI-XR-9720
Product Group: Secondary Antibody
Supplier: ProSci
CodeSizePrice
PSI-XR-9720-2mg2mg£440.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Peroxidase Conjugated Affinity Purified anti-Mouse IgG can be stored vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely

Documents

Further Information

Application Note:
Peroxidase Conjugated Affinity Purified anti-Mouse IgG is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:50,000 - 1:200,000, Western Blot 1:4,000 - 1:20,000, Immunohistochemistry 1:1,000 - 1:8,000. (Optimal dilution should be determined by user).
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/mL Bovine Serum Albumin (BSA); 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Conjugate:
HRP
Immunogen:
Mouse IgG - whole molecule
Physical State:
Lyophilized
Purification:
Peroxidase Conjugated Affinity Purified anti-Mouse IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins.
Specificity:
Minimum Cross reactivity to Human Serum Proteins