Biotin Goat Anti-Rabbit IgG (H&L) Antibody
| Code | Size | Price |
|---|
| PSI-XR-9940-2mg | 2mg | £409.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rabbit
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Biotin Conjugated Affinity Purified anti-Rabbit IgG can be stored vial at 4 °C prior to restoration. For extended storage mix product with 50% glycerol and then aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawi
Documents
Further Information
Application Note:
Biotin Conjugated Affinity Purified anti-Rabbit IgG is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2?-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:500,000 of the reconstitution concentration is suggested for this product. (Optimal dilution should be determined by user).
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% (w/v) Sodium Azide; pH 7.2; 10 mg/mL Bovine Serum Albumin
Conjugate:
Biotin
Immunogen:
Rabbit IgG - whole molecule
Physical State:
Lyophilized
Purification:
Biotin Conjugated Affinity Purified anti-Rabbit IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins.
Specificity:
Minimum Cross reactivity to Human Serum Proteins