Peroxidase Goat Anti-Rabbit IgG (H&L) Antibody

ProSci
Product Code: PSI-XR-9920
Product Group: Secondary Antibody
Supplier: ProSci
CodeSizePrice
PSI-XR-9920-2mg2mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rabbit
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Peroxidase Conjugated Affinity Purified anti-Rabbit IgG can be stored vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completel

Documents

Further Information

Application Note:
Peroxidase Conjugated Affinity Purified anti-Rabbit IgG is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:20,000 - 1:100,000, Western Blot 1:2,000 - 1:10,000, Immunohistochemistry 1:1,000 - 1:5,000. (Optimal dilution should be determined by user).
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/mL Bovine Serum Albumin (BSA); 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Conjugate:
HRP
Immunogen:
Rabbit IgG - whole molecule
Physical State:
Lyophilized
Purification:
Peroxidase Conjugated Affinity Purified anti-Rabbit IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins.
Specificity:
Minimum Cross reactivity to Human Serum Proteins