CD28 Antibody [CD28.2] (APC)
| Code | Size | Price |
|---|
| PSI-76-737-25tests | 25tests | £344.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CD28.2
Regulatory Status: RUO
Target Species: Human
Application: Flow Cytometry
Storage:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.
Documents
Further Information
Additional Names:
Tp44, CD28
Background:
The CD28.2 monoclonal antibody specifically binds with the human 44 kDa homodimeric trans-membrane glycoprotein CD28, expressed on the surface of most mature T lymphocytes, plasma cells, and thymocytes. CD28 is a ligand for B7-1 (CD80) and B7-2 (CD86), a co-stimulator of T lymphocytes, and enhances the interaction between T and B lymphocytes. It has been reported that the T lymphocytes stimulation to produce IL-2 depends on the monoclonal antibody involved, which suggests that the CD28 molecule presents some subregions with distinct functions. The CD28.2 antibody induces Ca2+ influx in Jurkat T lymphocytes. Other studies have shown that CD28 is involved in the signal transduction.
Background References:
- Nandi, D., Gross, J. A., Allison, J. P. (1994). CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells.The Journal of Immunology,152(7), 3361-3369.
- Gross, J. A., Callas, E. L. E. N. I., Allison, J. P. (1992). Identification and distribution of the costimulatory receptor CD28 in the mouse.The Journal of Immunology,149(2), 380-388.
- Grossi, J. A., Raulet, D. H., Allison, J. P. (1992). CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones.Nature,356, 607-9.
- Nishio, M., Spielman, J., Lee, R. K., Nelson, D. L., Podack, E. R. (1996). CD80 (B7. 1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis.The Journal of Immunology,157(10), 4347-4353.
Buffer:
Phosphate-buffered aqueous solution, ?0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Concentration:
batch dependent
Conjugate:
APC
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
NCBI Gene ID #:
920
NCBI Official Name:
CD28 molecule
NCBI Official Symbol:
CD28
NCBI Organism:
Homo sapiens
Physical State:
liquid
Purification:
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Research Area:
Immunology
SPECIFICITY:
The CD28.2 monoclonal antibody specifically binds with the human 44 kDa homodimeric trans-membrane glycoprotein CD28, expressed on the surface of most mature T lymphocytes, plasma cells, and thymocytes.
Swissprot #:
P10747
User NOte:
Optimal dilutions for each application to be determined by the researcher.