CD8a Antibody [RPA-T8] (APC-Cy7)
| Code | Size | Price |
|---|
| PSI-76-717-25tests | 25tests | £348.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: RPA-T8
Regulatory Status: RUO
Target Species: Human
Application: Flow Cytometry
Storage:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.
Documents
Further Information
Additional Names:
CD8, MAL, p32, Leu2, CD8A
Background:
The RPA-T8 monoclonal antibody specifically reacts with the human CD8a molecule, a 32 kDa cell surface receptor expressed either as a heterodimer (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha) on the majority of thymocytes, a subpopulation of mature T cells, and natural killer cells. CD8 interacts with the major histocompatibility complex class I (MHC class I) molecules on antigen-presenting cells or epithelial cells. The RPA-T8 antibody reacts with 13-48% of peripheral lymphocytes, 80% of thymocytes, and a subset of natural killer cells. RPA-T8, OKT8, and HIT8a antibodies do not compete with each other for binding to peripheral leukocytes, meaning that that they do not recognize the same epitope or block each other by steric hindrance.
Background References:
- Lefrancois, L. (1991). Phenotypic complexity of intraepithelial lymphocytes of the small intestine.The Journal of Immunology,147(6), 1746-1751.
- Walker, I. D., Murray, B. J., Hogarth, P. M., Kelso, A., McKenzie, I. F. (1984). Comparison of thymic and peripheral T cell Ly2/3 antigens.European journal of immunology,14(10), 906-910.
- THOMA-USZYNSKI, S., LADEL, C., KAUFMANN, S. (1997). Abscess formation in Listeria monocytogenes-infected gamma-delta T cell deficient mouse mutants involves alpha -beta T cells.Microbial pathogenesis,22(2), 123-128.
Buffer:
Phosphate-buffered aqueous solution, ?0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Concentration:
batch dependent
Conjugate:
APC-Cy7
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
NCBI Gene ID #:
925
NCBI Official Name:
CD8a molecule
NCBI Official Symbol:
CD8A
NCBI Organism:
Homo sapiens
Physical State:
liquid
Purification:
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Research Area:
Immunology
SPECIFICITY:
The RPA-T8 monoclonal antibody specifically reacts with the human CD8a molecule, a 32 kDa cell surface receptor expressed either as a heterodimer (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha) on the majority of thymocytes, a subpopulation of mature T cells, and natural killer cells.
Swissprot #:
P01732
User NOte:
Optimal dilutions for each application to be determined by the researcher.