CD86 Antibody [IT2.2] (PE)
| Code | Size | Price |
|---|
| PSI-76-595-100tests | 100tests | £601.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: IT2.2
Regulatory Status: RUO
Target Species: Human
Application: Flow Cytometry
Storage:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.
Documents
Further Information
Additional Names:
B70, B7-2, B7.2, LAB72, CD28LG2, CD86
Background:
The IT2.2 antibody reacts with human CD86, also known as B7-2, an 80 kDa cell surface protein which is a ligand for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 can also bind a second B7 ligand known as CD80 (B7-1). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.The IT2.2 antibody may be used as a marker for CD86 expression on B cells, macrophages, and dendritic cells. It is reported to be cross-reactive with Rhesus, Cynomolgus and Common marmoset CD86.
Background References:
- Hathcock, K. S., Laszlo, G., Dickler, H. B., Bradshaw, J., Linsley, P., Hodes, R. J. (1993). Identification of an alternative CTLA-4 ligand costimulatory for T cell activation.Science,262(5135), 905-907.
- Inaba, K., Witmer-Pack, M., Inaba, M., Hathcock, K. S., Sakuta, H., Azuma, M., ... Steinman, R. M. (1994). The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro.The Journal of experimental medicine,180(5), 1849-1860.
- Hathcock, K. S., Laszlo, G., Pucillo, C., Linsley, P., Hodes, R. J. (1994). Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function.The Journal of experimental medicine,180(2), 631-640.
Buffer:
Phosphate-buffered aqueous solution, ?0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Concentration:
batch dependent
Conjugate:
PE
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
NCBI Gene ID #:
942
NCBI Official Name:
CD86 molecule
NCBI Official Symbol:
CD86
NCBI Organism:
Homo sapiens
Physical State:
liquid
Purification:
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Research Area:
Innate Immunity
SPECIFICITY:
The IT2.2 antibody reacts with human CD86, also known as B7-2, an 80 kDa cell surface protein which is a ligand for CD28, a co-stimulatory receptor for the T cell receptor (TCR).
Swissprot #:
P42081
User NOte:
Optimal dilutions for each application to be determined by the researcher.