CD3 Antibody [Hit3a]

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ProSci
Product Code: PSI-76-213
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-76-213-0.1mg0.1mg£307.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: Hit3a
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Functional Study
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
Storage:
The product should be stored undiluted at 4°C. Do not freeze.

Documents

Further Information

Additional Names:
T3E, TCRE, IMD18, CD3E
Background:
The Hit3a monoclonal antibody specifically reacts with the epsilon chain of the CD3/T lymphocyte antigen receptor complex. The CD3 complex is part of the TCR complex, expressed by all mature T lymphocytes and by the thymocyte lineage. CD3 enhances the antigen recognition by signal transduction.The HIT3a antibody cross-links with the TCR complex, initiating the cellular activation and proliferation, but it cannot be used for intracellular CD3 staining, as it is able to stain only the surface CD3 complex.
Background References:
  • McMichael, A. J. (1987). Leucocyte typing III.Oxford University Press, Oxford. Norton AJ, Isaacson PG (1985)
  • Knapp W(1989) Leucocyte typing IV: white cell differentiation antigens. Oxford University Press, 1989.
  • Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York
  • Lanier, L. L., Allison, J. P., Phillips, J. H. (1986). Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation.The Journal of Immunology,137(8), 2501-2507.
Buffer:
Phosphate-buffered aqueous solution, ?0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
NCBI Gene ID #:
916
NCBI Official Name:
CD3e molecule, epsilon (CD3-TCR complex)
NCBI Official Symbol:
CD3E
NCBI Organism:
Homo sapiens
Physical State:
liquid
Purification:
The monoclonal antibody was purified utilizing affinity chromatography.
Research Area:
Immunology,Functional Assays
SPECIFICITY:
The Hit3a monoclonal antibody specifically reacts with the epsilon chain of the CD3/T lymphocyte antigen receptor complex.
Swissprot #:
P07766
User NOte:
Optimal dilutions for each application to be determined by the researcher.