RFX1 Antibody
| Code | Size | Price |
|---|
| PSI-8199-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8199-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
RFX1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
RFX, MHC class II regulatory factor RFX, MHC class II regulatory factor RFX1, enhancer factor C, EFC, trans-acting regulatory factor 1, transcription factor RFX1
Application Note:
RFX1 antibody can be used for detection of RFX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
RFX1 is a member of the regulatory factor X protein family, which are transcription factors that contain a highly-conserved winged helix DNA binding domain and the D region found in the C-terminal part of these proteins which facilitates dimerization (1,2). RFX1 is structurally related to regulatory factors X2, X3, X4, and X5 (3). RFX1 is a regulatory factor essential for MHC class II genes expression and can bind to an inverted repeat that is required for expression of hepatitis B virus genes (4). Recent study identifies RFX1 may play a tumor suppressor role in HCC as autophagy mediator (5).
Background References:
- Reith W, Ucla C, Barras E, et al. RFX1, a transactivator of hepatitis B virus enhancer I, belongs to a novel family of homodimeric and heterodimeric DNA-binding proteins. Mol. Cell Biol. 1994; 14:1230-44.
- Katan-Khaykovich Y and Shaul Y. RFX1, a single DNA-binding protein with a split dimerization domain, generates alternative complexes. J. Biol. Chem. 1998; 273:24504-12.
- Hsu YC, Liao WC, Kao CY, et al. Regulation of FGF1 gene promoter through transcription factor RFX1. J. Biol. Chem. 2010; 285:13885-95.
- Su JC, Tseng PH, Hsu CY, et al. RFX1-dependent activation of SHP-1 induces autophagy by a novel obatoclax derivative in hepatocellular carcinoma cells. Oncotarget 2014; 5:4909-19.
Buffer:
RFX1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (89%)
Immunogen:
RFX1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RFX1.
The immunogen is located within the last 50 amino acids of RFX1.
The immunogen is located within the last 50 amino acids of RFX1.
Molecular Weight Explanation:
multiple independent RFX1 antibodies also show a band at ~130 kDa
NCBI Gene ID #:
5989
NCBI Official Name:
regulatory factor X, 1 (influences HLA class II expression)
NCBI Official Symbol:
RFX1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 108 kDa
Observed: 125 kDa
Observed: 125 kDa
Protein Accession #:
NP_002909
Protein GI Number:
238859557
Purification:
RFX1 antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology,Autophagy
SPECIFICITY:
RFX1 antibody is human specific. At least two isoforms of RFX1 are known to exist; this antibody will detect both isoforms.
Swissprot #:
P22670
User NOte:
Optimal dilutions for each application to be determined by the researcher.