C/EBP-ε (phospho Thr74) Antibody

ProSci
Product Code: PSI-79-808
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-808-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from HUVEC cells treated with UV using C/EBP-ε (Phospho-Thr74) Antibody. The lane on the right is treated with the antigen-specific peptide.

Western blot analysis of extracts from HUVEC cells treated with UV using C/EBP-ε (Phospho-Thr74) Antibody. The lane on the right is treated with the antigen-specific peptide.

Further Information

Additional Names:
CEBPE, C/EBP epsilon, CCAAT/enhancer binding protein epsilon
Application Note:
Western Blot: 1:500~1:1000
Background:
C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
Background References:
  • Arati Khanna-Gupta. PNAS, Jul 2001; 98: 8000.
  • Dazhong Zhuang. J. Biol. Chem., Apr 2006; 281: 10745 - 10751.
  • Julie A. Lekstrom-Himes. Stem Cells, Mar 2001; 19: 125 - 133.
  • Ryuya Yamanaka. PNAS, Nov 1997; 94: 13187.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
C/EBP-ε (phospho Thr74) antibody was raised against a peptide sequence around phosphorylation site of threonine 174 (L-A-T (p)-A-A) derived from Human C/EBP-ε.
Modifications:
Phospho-Specific
NCBI Gene ID #:
1053
NCBI Official Name:
CCAAT/enhancer binding protein (C/EBP), epsilon
NCBI Official Symbol:
CEBPE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
NP_001796.2
Protein GI Number:
28872800
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of C/EBP-? only when phosphorylated at threonine 74
Swissprot #:
Q15744