IRF-3 (phospho Ser386) Antibody
| Code | Size | Price |
|---|
| PSI-79-871-0.1mg | 0.1mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.
Documents
Further Information
Additional Names:
IRF3, Interferon regulatory factor 3
Application Note:
Western Blot: 1:500~1:1000
Background:
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
Background References:
- Au W.W.-C., Proc. Natl. Acad. Sci. U.S.A. 92:11657-11661(1995).
- The MGC Project Team; Genome Res. 14:2121-2127(2004).
- Bellingham J., Ann. Hum. Genet. 62:231-234(1998).
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
IRF-3 (phospho Ser386) antibody was raised against a peptide sequence around phosphorylation site of Serine 386 (A-S-S (p)-L-E) derived from Human IRF-3.
Modifications:
Phospho-Specific
NCBI Gene ID #:
3661
NCBI Official Name:
interferon regulatory factor 3
NCBI Official Symbol:
IRF3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
55 kDa
Protein Accession #:
NP_001184052.1
Protein GI Number:
4504725
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of IRF-3 only when phosphorylated at serine 386.
Swissprot #:
Q14653