Arrestin 1 (phospho Ser412) Antibody

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ProSci
Product Code: PSI-79-784
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-784-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from COS7 cells treated with Etoposide using Arrestin 1 (Phospho-Ser412) Antibody. The lane on the right is treated with the antigen-specific peptide.
2 / 2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Arrestin 1 (Phospho-Ser412) antibody (left) or the same antibody preincubated with blocking peptide (right).
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Western blot analysis of extracts from COS7 cells treated with Etoposide using Arrestin 1 (Phospho-Ser412) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Arrestin 1 (Phospho-Ser412) antibody (left) or the same antibody preincubated with blocking peptide (right).

Documents

Further Information

Additional Names:
ARR1, arrestin 2, arrestin beta 1, beta-arrestin-1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Functions in regulating agonist-mediated G-protein coupled receptor (GPCR) signaling by mediating both receptor desensitization and resensitization processes. During homologous desensitization, beta-arrestins bind to the GPRK-phosphorylated receptor and sterically preclude its coupling to the cognate G-protein; the binding appears to require additional receptor determinants exposed only in the active receptor conformation.
Background References:
  • Greg Buchanan. AACR Meeting Abstracts, Apr 2006; 2006: 430.
  • D. Alex Groarke. Mol. Pharmacol., Feb 2001; 59: 375.
  • Karen McConalogue. Mol. Biol. Cell, Aug 1998; 9: 2305.
  • Maria Stella Lombardi. Stroke, Apr 2004; 35: 981 - 986.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Arrestin 1 (phospho Ser412) antibody was raised against a peptide sequence around phosphorylation site of Serine 412 (T-G-S (p)-P-Q) derived from Human Arrestin 1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
408
NCBI Official Name:
arrestin, beta 1
NCBI Official Symbol:
ARRB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
NP_004032.2
Protein GI Number:
10880136
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of Arrestin-1 only when phosphorylated at serine 412.
Swissprot #:
P49407