Bloom Syndrome (phospho Thr99) Antibody

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ProSci
Product Code: PSI-79-803
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-803-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from HepG2 cells using Bloom Syndrome Protein (Phospho-Thr99) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Immunohistochemical analysis of paraffin-embedded human heart tissue, using Bloom Syndrome Protein (Phospho-Thr99) antibody (left) or the same antibody preincubated with blocking peptide (right).
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Western blot analysis of extracts from HepG2 cells using Bloom Syndrome Protein (Phospho-Thr99) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human heart tissue, using Bloom Syndrome Protein (Phospho-Thr99) antibody (left) or the same antibody preincubated with blocking peptide (right).

Documents

Further Information

Additional Names:
RECQ2, RECQL3, type 2, EC 3.6.1, RecQ protein-like 3
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).
Background References:
  • Ellis N.A., Cell 83:655-666(1995).
  • Karow J.K., J. Biol. Chem. 272:30611-30614(1997).
  • Pichierri P., EMBO J. 23:3154-3163(2004).
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Bloom Syndrome (phospho Thr99) antibody was raised against a peptide sequence around phosphorylation site of threonine 99 (Q-E-T (p)-Q-R) derived from Human Bloom Syndrome.
Modifications:
Phospho-Specific
NCBI Gene ID #:
641
NCBI Official Name:
Bloom syndrome, RecQ helicase-like
NCBI Official Symbol:
BLM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
159 kDa
Protein Accession #:
NP_000048.1
Protein GI Number:
1705486
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of Bloom Syndrome Protein only when phosphorylated at threonine 99.
Swissprot #:
P54132