p47 phox (phospho Ser345) Antibody

ProSci
Product Code: PSI-79-920
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-920-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from HepG2 cells treated with TNF using p47 phox (Phospho-Ser345) Antibody. The lane on the right is treated with the antigen-specific peptide.
2 / 2
Immunohistochemical analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody (left) or the same antibody preincubated with blocking peptide (right).

Western blot analysis of extracts from HepG2 cells treated with TNF using p47 phox (Phospho-Ser345) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody (left) or the same antibody preincubated with blocking peptide (right).

Further Information

Additional Names:
NCF1, P47 phox, NCF-47K
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Background References:
  • Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989).
  • Lomax K.J., Science 245:409-412(1989).
  • Rodaway A.R.F., Mol. Cell. Biol. 10:5388-5396(1990).
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
p47 phox (phospho Ser345) antibody was raised against a peptide sequence around phosphorylation site of Serine 345 (P-Q-S (p)-P-G) derived from Human Nuclear p47 phox.
Modifications:
Phospho-Specific
NCBI Gene ID #:
653361
NCBI Official Name:
neutrophil cytosolic factor 1
NCBI Official Symbol:
NCF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
NP_000256.4
Protein GI Number:
115298672
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of p47 phox only when phosphorylated at serine 345.
Swissprot #:
P14598