CD227/MUC1 (phospho Tyr1229) Antibody

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ProSci
Product Code: PSI-79-825
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-825-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from HepG2 cells treated with PMA using CD227/MUC1 (Phospho-Tyr1229) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CD227/MUC1 (Phospho-Tyr1229) antibody (left) or the same antibody preincubated with blocking peptide (right).
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Western blot analysis of extracts from HepG2 cells treated with PMA using CD227/MUC1 (Phospho-Tyr1229) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CD227/MUC1 (Phospho-Tyr1229) antibody (left) or the same antibody preincubated with blocking peptide (right).

Documents

Further Information

Additional Names:
PUM, EMA, H23AG, MUC-1, PEM
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
Background References:
  • Lan M.S., J. Biol. Chem. 265:15294-15299(1990).
  • Ligtenberg M.J.L., J. Biol. Chem. 265:5573-5578(1990).
  • Gendler S.J., J. Biol. Chem. 265:15286-15293(1990)
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CD227/MUC1 (phospho Tyr1229) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 1229 S-P-Y (p)-E-K) derived from Human CD227/MUC1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
4582
NCBI Official Name:
mucin 1, cell surface associated
NCBI Official Symbol:
MUC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
170 kDa
Protein Accession #:
NP_001018016.1
Protein GI Number:
324120958
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of CD227/MUC1 only when phosphorylated at tyrosine 1229.
Swissprot #:
P15941