RAET1E Antibody

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ProSci
Product Code: PSI-8193
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8193-0.02mg0.02mg£150.00
Quantity:
PSI-8193-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
RAET1E antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

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Western blot analysis of RAET1E in EL4 cell lysate with RAET1E antibody at 1 μg/ml.
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Immunocytochemistry of RAET1E in K562 cells with RAET1E at 2.5 μg/mL.
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Western blot analysis of RAET1E in EL4 cell lysate with RAET1E antibody at 1 μg/ml.
Immunocytochemistry of RAET1E in K562 cells with RAET1E at 2.5 μg/mL.

Documents

Further Information

Additional Names:
Retinoic acid early transcript 1E, NKG2D ligand 4, N2DL-4, NKG2DL42, RL-4, ULBP4, LETAL
Application Note:
RAET1E antibody can be used for detection of RAET1E by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
The Retinoic acid early transcript 1E (RAET1E) belongs to the RAET1 family, which consists of major histocompatibility complex (MHC) class I-related genes located in a cluster on chromosome 6q24.2-q25.3 (1). Like the related protein RAET1G, RAET1E differs from other RAET1 proteins in that they have type I membrane-spanning sequences at their carboxy termini rather than glycosylphosphatidylinositol anchor sequences (2). RAET1E functions as a ligand for NKG2D receptor, which is expressed on the surface of several types of immune cells, and is involved in innate and adaptive immune responses (1,3).
Background References:
  • Radosavlijevic M, Cuillerier B, Wilson MJ, et al. A cluster of ten novel MHC class I related genes on human chromosome 6q24.2-q25.3. Genomics 2002; 79:114-23.
  • Bacon L, Eagle RA, Meyer M, et al. Two human ULBP/RAET1 molecules with transmembrane regions are ligands for NKG2D. J. Immunol. 2004; 173:1078-84.
  • Letal, a tumor-associated NKG2D immunoreceptor ligand, induces activation and expansion of effector immune cells. Cancer Biol. Ther. 2003; 2:446-51.
Buffer:
RAET1E antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
RAET1E antibody was raised against an 18 amino acid peptide near the center of human RAET1E.

The immunogen is located within amino acids 160 - 210 of RAET1E.
NCBI Gene ID #:
135250
NCBI Official Name:
retinoic acid early transcript 1E
NCBI Official Symbol:
RAET1E
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 29 kDa

Observed: 29 kDa
Protein Accession #:
NP_631904
Protein GI Number:
21040249
Purification:
RAET1E antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity,Cancer
SPECIFICITY:
RAET1E antibody is human and mouse reactive. At least four isoforms of RAET1E are known to exist; this antibody will detect all four.
Swissprot #:
P84103
User NOte:
Optimal dilutions for each application to be determined by the researcher.