UBAP2L Antibody

ProSci
Product Code: PSI-8371
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8371-0.02mg0.02mg£150.00
Quantity:
PSI-8371-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
UBAP2L antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

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Western blot analysis of UBAP2L in HeLa cell lysate with UBAP2L antibody at 1 μg/ml.
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Immunocytochemistry of UBAP2L in HeLa cells with UBAP2L antibody at 10 μg/ml.
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Immunofluorescence of UBAP2L in HeLa cells with UBAP2L antibody at 20 μg/ml.<br><br>Red: UBAP2L Antibody (8371) <br> Blue: DAPI staining

Western blot analysis of UBAP2L in HeLa cell lysate with UBAP2L antibody at 1 μg/ml.
Immunocytochemistry of UBAP2L in HeLa cells with UBAP2L antibody at 10 μg/ml.
Immunofluorescence of UBAP2L in HeLa cells with UBAP2L antibody at 20 μg/ml.<br><br>Red: UBAP2L Antibody (8371) <br> Blue: DAPI staining

Further Information

Additional Names:
Ubiquitin associated protein 2-like, NICE-4
Application Note:
UBAP2L antibody can be used for the detection of UBAP2L by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry at 10 μg/ml.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The UBA domain-containing protein ubiquitin associated protein 2-like (UBAP2L), also known as NICE4, is a ubiquitously expressed 1,087 amino acid protein. UBAP2L is preferentially expressed in hematopoietic stem cell (HSC) populations and interacts with BMI1, a well-known determinant of HSC function (1). It is essential for the activity of long-term repopulating HSCs but not the BMI1-dependent repression of the Ink4a/Arf locus (1).
Background References:
  • Bordeleau ME, Aucagne R, Chagraoui J, et al. UBAPL2L is a novel BMI1-iteracting protein essential for hematopoietic stem cell activity. Blood 2014; 124:2362-9.
Buffer:
UBAP2L Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
UBAP2L antibody was raised against a 19 amino acid peptide from near the amino terminus of human UBAP2L.

The immunogen is located within amino acids 200 - 250 of UBAP2L.
NCBI Gene ID #:
9898
NCBI Official Name:
ubiquitin associated protein 2-like
NCBI Official Symbol:
UBAP2L
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 120 kDa

Observed: 130 kDa
Protein Accession #:
NP_055662
Protein GI Number:
188497758
Purification:
UBAP2L Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
UBAP2L antibody is human and mouse reactive. At least four isoforms of UBAP2L are known to exist; this antibody will detect all four isoforms. This antibody is predicted to not cross-react with UBAP2.
Swissprot #:
Q14157
User NOte:
Optimal dilutions for each application to be determined by the researcher.