ATF1 (phospho Ser63) Antibody

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ProSci
Product Code: PSI-79-965
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-965-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Application: Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from HT29 cells treated with Insulin using Phospho-ATF1 (Ser63) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Western blot analysis of extracts from HT29 cells treated with Insulin using Phospho-ATF1 (Ser63) Antibody. The lane on the right is treated with the antigen-specific peptide.

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Further Information

Additional Names:
ATF1, Activating transcription factor 1, Cyclic-AMP-dependent transcription factor ATF-1, TCR-ATF1, TREB36 protein
Application Note:
Western Blot: 1:500~1:1000
Background:
This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.
Background References:
  • Mackenzie KF, et al. (2013) J Immunol 190, 565-77
  • Dickinson RJ, et al. (2011) J Biol Chem 286, 38018-26
  • Wang J, Zhou JY, Wu GS (2011) J Biol Chem 286, 22384-92
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ATF1 (phospho Ser63) antibody was raised against a peptide sequence around phosphorylation site of serine 63 (R-P-S (p)-Y-R) derived from Human ATF1 .
Modifications:
Phospho-Specific
NCBI Gene ID #:
466
NCBI Official Name:
activating transcription factor 1
NCBI Official Symbol:
ATF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
29 kDa
Protein Accession #:
NP_005162.1
Protein GI Number:
4885073
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous level of ATF1 only when phosphorylated at serine 63.
Swissprot #:
P18846