Estrogen Receptor-alpha (phospho Tyr537) Antibody

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ProSci
Product Code: PSI-79-786
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-786-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from K562 cells (Lane 2), using Estrogen Receptor-alpha (Phospho-Tyr537) Antibody. The lane on the left is treated with antigen-specific peptide.
2 / 2
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Estrogen Receptor-alpha (Phospho-Tyr537) antibody (left) or the same antibody preincubated with blocking peptide (right).
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Western blot analysis of extracts from K562 cells (Lane 2), using Estrogen Receptor-alpha (Phospho-Tyr537) Antibody. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Estrogen Receptor-alpha (Phospho-Tyr537) antibody (left) or the same antibody preincubated with blocking peptide (right).

Documents

Further Information

Additional Names:
ER, ESR, ESR1, ESTR, NR3A1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
Background References:
  • Sehime Temel, Endocrinology, Oct 2002; 143: 3974.
  • Xin-Tian Zhang, AACR Meeting Abstracts, Apr 2005; 2005: 302.
  • Suwit J. Somponpun, Am J Physiol Regulatory Integrative Comp Physiol, Sep 2004; 287: R661 - R669.
  • Marcello Maggiolini, Cancer Res., Oct 1999;
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Estrogen Receptor-alpha (phospho Tyr537) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 537 (P-L-Y (p)-D-L) derived from Human Estrogen Receptor-alpha.
Modifications:
Phospho-Specific
NCBI Gene ID #:
2099
NCBI Official Name:
estrogen receptor 1
NCBI Official Symbol:
ESR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66 kDa
Protein Accession #:
NP_000116.2
Protein GI Number:
62821794
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of ER-alpha only when phosphorylated at tyrosine 537.
Swissprot #:
P03372