FADD (phospho Ser191) Antibody

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ProSci
Product Code: PSI-79-929
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-929-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.

Images

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Western blot analysis of extracts from Jurkat cells treated with PMA using FADD (Phospho-Ser191) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using FADD (Phospho-Ser191) antibody (left) or the same antibody preincubated with blocking peptide (right).
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Western blot analysis of extracts from Jurkat cells treated with PMA using FADD (Phospho-Ser191) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using FADD (Phospho-Ser191) antibody (left) or the same antibody preincubated with blocking peptide (right).

Documents

Further Information

Additional Names:
MORT1 , FAS-associating death domain-containing protein, Mediator of receptor induced toxicity
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.
Background References:
  • Sugano S., Nat. Genet. 36:40-45(2004).
  • Farmer A., Submitted (MAY-2003).
  • Venter J.C., Submitted (JUL-2005).
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
FADD (phospho Ser191) antibody was raised against a peptide sequence around phosphorylation site of Serine191 (R-S-G (p)-A-M) derived from Human FADD.
Modifications:
Phospho-Specific
NCBI Gene ID #:
14082
NCBI Official Name:
Fas (TNFRSF6)-associated via death domain
NCBI Official Symbol:
FADD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
25 kDa
Protein Accession #:
NP_003815.1
Protein GI Number:
6753812
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of FADD only when phosphorylated at serine 191.
Swissprot #:
Q13158