ACK1 (phospho Tyr284) Antibody
| Code | Size | Price |
|---|
| PSI-79-795-0.1mg | 0.1mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store antibody at -20°C for up to one year.
Documents
Further Information
Additional Names:
ACK1, Activated p21cdc42Hs kinase, EC 2.7.10.2, kinase ACK1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes.
Background References:
- Manser E., Nature 363:364-367(1993).
- Ota T., Nat. Genet. 36:40-45(2004).
- Eisenmann K.M., Nat. Cell Biol. 1:507-513(1999)
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ACK1 (phospho Tyr284) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 284 (D-H-Y (p)-V-M) derived from Human ACK1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
10188
NCBI Official Name:
tyrosine kinase, non-receptor, 2
NCBI Official Symbol:
TNK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
120 kDa
Protein Accession #:
NP_005772.3
Protein GI Number:
229462980
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous levels of ACK1 only when phosphorylated at tyrosine 284.
Swissprot #:
Q07912