S1PR2 Antibody
| Code | Size | Price |
|---|
| PSI-8229-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8229-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
S1PR2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Sphingosine 1-phosphate receptor 2, S1P2, AGR16, EDG-5, EDG5, Gpcr13, H218, LPB2
Application Note:
S1PR2 antibody can be used for detection of S1PR2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry at 10 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
The Sphingosine 1-phosphate receptor 2 (S1PR2) protein was initially identified as G-protein coupled receptor thought to be involved in development (1) and was found to mediate sphingosine 1-phosphate (SPP)-induced cell rounding and neurite retraction (2). S1PR2 and the related protein S1PR3 (also known as EDG3) are also thought to mediate the SPP-induced cell proliferation, survival, and related signaling events (3). S1PR2 has also been found to be key regulator in acute vascular inflammation and may be a novel therapeutic target for vascular disorders (4).
Background References:
- MacLennan AJ, Browe CS, Gaskin AA, et al. Cloning and characterization of a putative G-protein coupled receptor potentially involved in development. Mol. Cell Neurosci. 1994; 5:201-9.
- Van Brocklyn JR, Tu Z, Edsall LC, et al. Sphingosine 1-phosphate-induced cell rounding and neurite retraction are mediated by the G protein-coupled receptor H218. J. Biol. Chem. 1999; 274:4626-32.
- An S, Zheng Y, and Bleu T. Sphingosine 1-phosphate-induced cell proliferation, survival, and related signaling events mediated by G protein-coupled receptors Edg3 and Edg5. J. Biol. Chem. 275:288-96.
- Zhang G, Yang L, Kim GS, et al. Critical role of sphingosine-1-phosphate receptor 2 (S1PR2) in acute vascular inflammation. Blood 2013; 122:443-55.
Buffer:
S1PR2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
S1PR2 antibody was raised against an 16 amino acid peptide near the carboxy terminus of human S1PR2.
The immunogen is located within amino acids 270 - 320 of S1PR2.
The immunogen is located within amino acids 270 - 320 of S1PR2.
Molecular Weight Explanation:
multiple transmembrane protein, also multiple other S1PR2 antibodies also show a ~50kD band
NCBI Gene ID #:
9294
NCBI Official Name:
sphingosine-1-phosphate receptor 2
NCBI Official Symbol:
S1PR2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 39 kDa
Observed: 50 kDa
Observed: 50 kDa
Protein Accession #:
NP_004221
Protein GI Number:
134244587
Purification:
S1PR2 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell,Signal Transduction,Immunology
SPECIFICITY:
S1PR2 antibody is human, mouse and rat reactive.
Swissprot #:
O95136
User NOte:
Optimal dilutions for each application to be determined by the researcher.