LRRC8A Antibody

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ProSci
Product Code: PSI-8235
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8235-0.02mg0.02mg£150.00
Quantity:
PSI-8235-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
LRRC8A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

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Western blot analysis of LRRC8A in EL4 cell lysate with LRRC8A antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of LRRC8A in rat spleen tissue with LRRC8A antibody at 2.5 μg/mL.
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Western blot analysis of LRRC8A in EL4 cell lysate with LRRC8A antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of LRRC8A in rat spleen tissue with LRRC8A antibody at 2.5 μg/mL.

Documents

Further Information

Additional Names:
Leucine rich repeat containing 8 family member A, leucine-rich repeat-containing protein 8A, LRRC8, AGM5
Application Note:
LRRC8A antibody can be used for detection of LRRC8A by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Background:
The leucine rich repeat containing 8 family member A protein (LRRC8A) belongs the LRRC8 family of proteins within the LRR superfamily of proteins, which are involved in diverse biological processes, including cell adhesion, cellular trafficking, and hormone-receptor interactions (1,2). LRRC8A is a four-pass transmembrane protein that plays a role in B cell development. Defects in this gene cause autosomal dominant non-Bruton type agammaglobulinemia, an immunodeficiency disease resulting from defects in B cell maturation (1). LRRC8A has also been shown to be an essential component of the volume-regulated anion channel VRAC (3).
Background References:
  • Sawada A, Takihara Y, Kim JY, et al. A congenital mutation of the novel gene LRRC8 causes agammaglobulinemia in humans. J. Clin. Invest. 2003; 112:1707-13.
  • Kubota K, Kim JY, Sawada A, et al. LRRC8 involved in B cell development belongs to a novel family of leucine-rich repeat proteins. FEBS Lett. 2004; 564:147-52.
  • Voss FK, Ullrich F, Munch J, et al. Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC. Science 2014; 344:634-8.
Buffer:
LRRC8A antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
LRRC8A antibody was raised against a 15 amino acid peptide near the amino terminus of human LRRC8A.

The immunogen is located within amino acids 60 - 110 of LRRC8A.
Molecular Weight Explanation:
multiple other LRRC8A antibodies also show a ~95kD band
NCBI Gene ID #:
56262
NCBI Official Name:
leucine rich repeat containing 8 family, member A
NCBI Official Symbol:
LRRC8A
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 89 kDa

Observed: 95 kDa
Protein Accession #:
NP_062540
Protein GI Number:
62241040
Purification:
LRRC8A antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell,Signal Transduction
SPECIFICITY:
LRRC8A antibody is human, mouse and rat reactive.
Swissprot #:
Q8IWT6
User NOte:
Optimal dilutions for each application to be determined by the researcher.