PMEPA1 Antibody

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ProSci
Product Code: PSI-8261
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8261-0.02mg0.02mg£150.00
Quantity:
PSI-8261-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
PMEPA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

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Western blot analysis of PMEPA1 inA549 cell lysate with PMEPA1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of PMEPA1 in human prostate tissue with PMEPA1 antibody at 2.5 μg/ml.
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Western blot analysis of PMEPA1 inA549 cell lysate with PMEPA1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of PMEPA1 in human prostate tissue with PMEPA1 antibody at 2.5 μg/ml.

Documents

Further Information

Additional Names:
Prostate transmembrane protein androgen induced 1, STAG1, TMEPAI
Application Note:
PMEPA1 antibody can be used for detection of PMEPA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
The prostate transmembrane protein, androgen induced 1 (PMEPA1) protein is a transmembrane protein that contains a Smad interacting motif (SIM) (1,2). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins (3). Overexpression of this gene may play a role in multiple types of cancer (2,4).
Background References:
  • Xu LL, Shanmugam N, Segawa T, et al. A novel androgen-regulated gene, PMEPA1, located on chromosome 20q!3 exhibits high level expression in prostate. Genomics 2000; 66:257-63.
  • Rae FK, Hooper JD, Nicol DL, et al. Characterization of a novel gene, STAG1/PMEPA1, upregulated in renal cell carcinoma and other solid tumors. Mol. Carcinog. 2001; 32:44-53.
  • Watanabe Y, Itoh S, Goto T, et al. TMEPAI, a transmembrane TGF-beta-inducible protein, sequesters Smad proteins from active participation in TGF-beta signaling. Mol. Cell 2010; 37:123-34.
  • Vo Nguyen TT, Watanabe Y, Shiba A, et al. TMEPAI/PMEPA1 enhances tumorigenic activities in lung cancer cells. Cancer Sci. 2014; 105:334-41.
Buffer:
PMEPA1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PMEPA1 antibody was raised against a 16 amino acid peptide near the center of human PMEPA1.

The immunogen is located within amino acids 160 - 210 of PMEPA1.
NCBI Gene ID #:
56937
NCBI Official Name:
prostate transmembrane protein, androgen induced 1
NCBI Official Symbol:
PMEPA1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 26, 28, 32 kDa

Observed: 27 kDa
Protein Accession #:
NP_064567
Protein GI Number:
21361841
Purification:
PMEPA1 antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Signal Transduction
SPECIFICITY:
PMEPA1 antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect all of the isoforms.
Swissprot #:
Q969W9
User NOte:
Optimal dilutions for each application to be determined by the researcher.