TRPC3 Antibody
| Code | Size | Price |
|---|
| PSI-8269-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8269-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
TRPC3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
TRP3, TRP3, Short transient receptor potential channel 3, Transient receptor protein 3, TrpC3
Application Note:
TRPC3 antibody can be used for the detection of TRPC3 by Western blot at 1 - 2 μg/mL.Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP" (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC3/6/7 subfamily can be activated by diacylglycerol analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. TRPC3 encodes a Ca2+-permeant channel that is agonist-activated but not store-operated or directly receptor-activated. TRPC3 physically interacts with TRPC6 and TRPC7 and forms functional tetrameric channels.
Background References:
- Contell C, Birnbaumer V, Flockerzi V, et al. A unified nomenclature for the superfamily of TRP cation channels. Mol. Cell 2002; 9:229-31.
- Trebak M, Vazquez G, Bird GSJ, et al. The TRPC3/6/7 subfamily of cation channels. Cell Calcium 2003; 33:451-61.
- Zitt C, Obukhov AG, Strubing C, et al. Expression of TRPC3 in Chinese hamster ovary cells results in calcium-activated cation currents not related to store depletion. J. Cell. Biol. 1997; 1333-41.
- Dietrich A, Kalwa H, Rost BR, et al. The diacylglycerol-sensitive TRPC3/6/7 subfamily of cation channels: functional characterization and physiological relevance. Pflugers Arch. 2005; 451:72-80.
Buffer:
TRPC3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TRPC3 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human TRPC3.
The immunogen is located within amino acids 710 - 760 of TRPC3.
The immunogen is located within amino acids 710 - 760 of TRPC3.
NCBI Gene ID #:
7222
NCBI Official Name:
transient receptor potential cation channel, subfamily C, member 3
NCBI Official Symbol:
TRPC3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 90, 93, 101 kDa
Observed: 93 kDa
Observed: 93 kDa
Protein Accession #:
NP_001124170
Protein GI Number:
194733735
Purification:
TRPC3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Neuroscience
SPECIFICITY:
TRPC3 antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect the three largest isoforms. TRPC3 antibody is predicted to not cross-react with TRPC6.
Swissprot #:
Q13507
User NOte:
Optimal dilutions for each application to be determined by the researcher.