ERp72 Antibody

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ProSci
Product Code: PSI-8211
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8211-0.02mg0.02mg£150.00
Quantity:
PSI-8211-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
ERp72 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

1 / 3
Western blot analysis of ERp72 in human colon tissue lysate with ERp72 antibody at (A) 1 and (B) 2 μg/ml.
2 / 3
Immunohistochemistry of ERp72 in rat colon tissue with ERp72 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of ERp72 in rat colon tissue with ERp72 antibody at 20 μg/mL.
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Western blot analysis of ERp72 in human colon tissue lysate with ERp72 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of ERp72 in rat colon tissue with ERp72 antibody at 5 μg/mL.
Immunofluorescence of ERp72 in rat colon tissue with ERp72 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
Protein disulfide isomerase family A member 4, Endoplasmic reticulum resident protein 70, Endoplasmic reticulum resident protein 72, ERp70, ERp-72
Application Note:
ERp72 antibody can be used for detection of ERp72 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
Protein disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (1,2). ER stress protein 72 (ERp72), also designated protein disulfide-isomerase A4, is involved in the catalysis of protein -S-S- bond rearrangement (3). It plays a role in the formation and isomerization of disulfide bonds (3,4).
Background References:
  • Huppa JB and Ploegh HL. The eS-Sence of -SH in the ER. Cell 1998; 92:145-8.
  • Ellgaard L and Ruddock LW. The human protein disulphide isomerase family: substrate interactions and functional properties. EMBO Rep. 2005; 6:28-32.
  • Mazzarella RA, Srinivasan M, Haugejorden SM, et al. ERp72, an abundant luminal endoplasmic reticulum protein, contains three copies of the active site sequences of protein disulfide isomerase. J. Biol. Chem. 1990; 265:1094-101.
  • Satoh M, Shimada A, Keino H, et al. Functional characterization of 3 thioredoxin homology domains of ERp72. Cell Stress Chaperones 2005; 10:278-84.
Buffer:
ERp72 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (80%)
Immunogen:
ERp72 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human ERp72.

The immunogen is located within the last 50 amino acids of ERp72.
NCBI Gene ID #:
9601
NCBI Official Name:
protein disulfide isomerase family A, member 4
NCBI Official Symbol:
PDIA4
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 71 kDa

Observed: 75 kDa
Protein Accession #:
NP_004902
Protein GI Number:
4758304
Purification:
ERp72 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
ERP72 antibody is human, mouse and rat reactive.
Swissprot #:
P13667
User NOte:
Optimal dilutions for each application to be determined by the researcher.