GOLGIN97 Antibody
| Code | Size | Price |
|---|
| PSI-8215-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8215-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
GOLGIN97 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Golgin subfamily A member 1, GOLGA1, GOLGIN A1, GOLGIN-97
Application Note:
GOLGIN97 antibody can be used for detection of GOLGIN97 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
GOLGIN97 was initially isolated as a Golgi complex autoantigen associated with Sjogren?s syndrome, a chronic autoimmune disease in which the body's white blood cells destroy the exocrine glands, specifically the salivary and lacrimal glands (1,2). GOLGIN97 plays a role as a tethering molecule in endosome to the trans-Golgi network (TGN) retrograde traffic (3). FIP1/RCP binding to GOLGIN97 is required for tethering and fusion of recycling endosome retrograde transport vesicles to the TGN (4).
Background References:
- Griffith KJ, Chan EK, Lung CC, et al. Molecular cloning of a novel 97-kd Golgi complex autoantigen associated with Sjogren's syndrome. Arthritis Rheum. 1997; 40:1693-702.
- Borchers AT, Naguwa SM, Keen CL, et al. Immunopathogenesis of Sjogren's syndrome. Clin. Rev. Allergy Immunol. 2003; 25:89-104.
- Lu L, Tai G, and Hong W. Autoantigen Golgin-97, and effector of ARL1 GTPase, participates in traffic from the endosome to the trans-Golgi network. Mol. Biol. Cell 2004; 15:4426-43.
- Jing J, Junutula JR, Wu C, et al. FIP1/RCP binding to Golgin-97 regulates retrograde transport from recycling endosomes to the trans-Golgi network. Mol. Biol. Cell 2010; 21:3041-53.
Buffer:
GOLGIN97 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
GOLGIN97 antibody was raised against a 17 amino acid peptide near the amino terminus of human GOLGIN97.
The immunogen is located within amino acids 30 - 80 of GOLGIN97.
The immunogen is located within amino acids 30 - 80 of GOLGIN97.
Molecular Weight Explanation:
heavily post-translationally modifed, multiple independent GOLGIN97 antibodies show bands at ~120kDa
NCBI Gene ID #:
2800
NCBI Official Name:
golgin A1
NCBI Official Symbol:
GOLGA1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 84 kDa
Observed: 120 kDa
Observed: 120 kDa
Protein Accession #:
NP_002068
Protein GI Number:
4504063
Purification:
GOLGIN97 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Immunology
SPECIFICITY:
GOLGIN97 antibody is human, mouse and rat reactive. At least two isoforms of GOLGIN97 are known to exist; this antibody will detect only the longer isoform. GOLGIN97 antibody is predicted to not cross-react with other GOLGIN family proteins.
Swissprot #:
Q92805
User NOte:
Optimal dilutions for each application to be determined by the researcher.