Hexokinase 1 Antibody
| Code | Size | Price |
|---|
| PSI-8217-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8217-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Hexokinase 1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
HK, HXK
Application Note:
Hexokinase 1 antibody can be used for detection of Hexokinase 1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
There are four major glucose-phosphorylating isoenzymes, designated Hexokinase 1 I, II, III, and IV (1). Hexokinase 1 activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway (1,2). Hexokinase 1 2 is the predominant Hexokinase 1 isozyme expressed in insulin-responsive tissues such as skeletal muscle and its expression is insulin-responsive (3). It is involved in the increased rate of glycolysis seen in rapidly growing cancer cells (4).
Background References:
- Chen T, Ning D, Sun H, et al. Sequence Analysis and Molecular Characterization of Clonorchis sinensis Hexokinase 1, an Unusual Trimeric 50-kDa Glucose-6-Phosphate-Sensitive Allosteric Enzyme. PLoS One 2014; 9:e107940.
- Halestrap AP, Pereira GC, and Pasdois P. The role of Hexokinase 1 in cardioprotection-mechanism and potential for translation. Br. J. Pharmacol. 2014; epub.
- Roberts DJ and Miyamoto S. Hexokinase 1 II integrates energy metabolism and cellular protection: Akting on mitochondria and TORCing to autophagy. Cell Death Differ. 2014; epub.
- Wang L, Xiong H, Wu F, et al. Hexokinase 1 2-Mediated Warburg Effect Is Required for PTEN- and p53-Deficiency-Driven Prostate Cancer Growth. Cell Rep. 2014; 8:1461-74.
Buffer:
Hexokinase 1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
Hexokinase 1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human Hexokinase 1.
The immunogen is located within amino acids 830 - 880 of Hexokinase 1.
The immunogen is located within amino acids 830 - 880 of Hexokinase 1.
NCBI Gene ID #:
3098
NCBI Official Name:
hexokinase 1
NCBI Official Symbol:
HK1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 101 kDa
Observed: 103 kDa
Observed: 103 kDa
Protein Accession #:
NP_000179
Protein GI Number:
188497754
Purification:
Hexokinase 1 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
Hexokinase 1 antibody is human, mouse and rat reactive. Multiple isoforms of Hexokinase 1 are known to exist.
Swissprot #:
P19367
User NOte:
Optimal dilutions for each application to be determined by the researcher.