LSD1 Antibody
| Code | Size | Price |
|---|
| PSI-8223-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8223-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
LSD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Lysine-specific histone demethylase, amine oxidase flavin containing domain protein 2, AOF2
Application Note:
WB: 1 - 2 μg/mL; IHC-P: 5 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction (1). Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription (2). LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation (3,4). Experiments have shown that CoREST, a SANT domain-containing corepressor (5) acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein (6), inhibits CoREST/LSD1 activity in vitro (3). LSD1-mediated histone demethylation thus may have significant effects on gene expression.
Background References:
- Shi et al. Cell 2004; 119:941-53.
- Kouzarides et al. Curr. Opin. Genet. Dev. 2002; 12:198-209.
- Shi et al. Mol. Cell 2005; 19:857-64.
- Nakamura et al. Mol. Cell 2002; 10:1119-28.
Buffer:
LSD1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anti-LSD1 antibody (8223) was raised against a peptide corresponding to 19 amino acids near the amino terminus of human LSD1.
The immunogen is located within amino acids 90-140 of LSD1.
The immunogen is located within amino acids 90-140 of LSD1.
ISOFORMS:
Human LSD1 has 2 isoforms, including isoform 1 (852aa, 93kD) and isoform 2 (876aa, 95kD). Mouse LSD1 has one isoform (853aa, 93kD) and Rat LSD1 also has one isoform (872aa, 95kD). 8223 can detect LSD1 in human, mouse and rat.
NCBI Gene ID #:
23028
NCBI Official Name:
lysine (K)-specific demethylase 1 A
NCBI Official Symbol:
KDM1A
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 93kD
Observed: 93 kD
Observed: 93 kD
Protein Accession #:
NP_001009999
Protein GI Number:
58761544
Purification:
LSD1 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
At least two isoforms of LSD1 are known to exist; this antibody detects both.
Swissprot #:
O60341
User NOte:
Optimal dilutions for each application to be determined by the researcher.